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Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
29/09/2023 |
Data da última atualização: |
29/09/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BRAGA, J. G. B.; NOVAIS, C. B. de; DINIZ, P. P.; ARAGÃO, O. O. da S.; SAGGIN JUNIOR, O. J.; JESUS, E. da C. |
Afiliação: |
JOÃO GABRIEL BARBOSA BRAGA, UFLA; CÂNDIDO BARRETO DE NOVAIS, UFRRJ; PRISCILA PEREIRA DINIZ, UFLA; OSNAR OBEDE DA SILVA ARAGÃO, UFLA; ORIVALDO JOSE SAGGIN JUNIOR, CNPAB; EDERSON DA CONCEICAO JESUS, CNPAB. |
Título: |
Association of mycoheterotrophic Gentianaceae with specific Glomus lineages. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Mycorrhiza, v. 33, n. 4, p. 249 - 256, July 2023. |
Idioma: |
Inglês |
Conteúdo: |
Some plant species took an alternative evolutionary pathway in which they lost their photosynthetic capacity to depend exclusively on carbon supplied by arbuscular mycorrhizal fungi (AMF) in an association called mycoheterotrophy. Among them is Voyriella parviflora, a species of the family Gentianaceae, which is found in tropical regions such as the Amazon basin. Here, we assessed the identity of AMF symbionts associated with this species. DNA was isolated from eight Gentianaceae specimens and from litter and surrounding roots of photosynthetic plants. The atp1 gene was amplified by Sanger sequencing to determine the taxonomic affiliation of the mycoheterotrophic plants. A 280 bp region of the 18S rRNA gene of AMF was amplified with primers NS31/AML2 by high-throughput sequencing. The mycoheterotrophic specimens were assigned to V. parviflora with a bootstrap support of 72%. Glomus was the most abundant AMF genus, both in the mycoheterotrophic plants and in the litter and roots of photosynthetic plants. In addition, a few Glomus genotypes were abundantly enriched in the mycoheterotrophic plants, with only a few specimens colonized by Gigaspora, Acaulospora, and Scutellospora in a low proportion. These genotypes formed a cluster within a larger clade, suggesting that V. parviflora shows a preferential association with a narrow Glomus lineage which is not phylogenetically close to a previously identified V. parviflora?s associated lineage. Furthermore, detecting fungi from other families suggests that V. parviflora is colonized by other genera, although with low frequency. These findings provide new insights into the association between AMF and mycoheterotrophic species and highlight the importance of considering trap culture-independent approaches in understanding this symbiosis MenosSome plant species took an alternative evolutionary pathway in which they lost their photosynthetic capacity to depend exclusively on carbon supplied by arbuscular mycorrhizal fungi (AMF) in an association called mycoheterotrophy. Among them is Voyriella parviflora, a species of the family Gentianaceae, which is found in tropical regions such as the Amazon basin. Here, we assessed the identity of AMF symbionts associated with this species. DNA was isolated from eight Gentianaceae specimens and from litter and surrounding roots of photosynthetic plants. The atp1 gene was amplified by Sanger sequencing to determine the taxonomic affiliation of the mycoheterotrophic plants. A 280 bp region of the 18S rRNA gene of AMF was amplified with primers NS31/AML2 by high-throughput sequencing. The mycoheterotrophic specimens were assigned to V. parviflora with a bootstrap support of 72%. Glomus was the most abundant AMF genus, both in the mycoheterotrophic plants and in the litter and roots of photosynthetic plants. In addition, a few Glomus genotypes were abundantly enriched in the mycoheterotrophic plants, with only a few specimens colonized by Gigaspora, Acaulospora, and Scutellospora in a low proportion. These genotypes formed a cluster within a larger clade, suggesting that V. parviflora shows a preferential association with a narrow Glomus lineage which is not phylogenetically close to a previously identified V. parviflora?s associated lineage. Furthermore, detecting fungi from oth... Mostrar Tudo |
Palavras-Chave: |
Amazon; Metabarcoding; Mycoheterotrophy; Rbuscular mycorrhizal fungi. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02480naa a2200229 a 4500 001 2156980 005 2023-09-29 008 2023 bl uuuu u00u1 u #d 100 1 $aBRAGA, J. G. B. 245 $aAssociation of mycoheterotrophic Gentianaceae with specific Glomus lineages.$h[electronic resource] 260 $c2023 520 $aSome plant species took an alternative evolutionary pathway in which they lost their photosynthetic capacity to depend exclusively on carbon supplied by arbuscular mycorrhizal fungi (AMF) in an association called mycoheterotrophy. Among them is Voyriella parviflora, a species of the family Gentianaceae, which is found in tropical regions such as the Amazon basin. Here, we assessed the identity of AMF symbionts associated with this species. DNA was isolated from eight Gentianaceae specimens and from litter and surrounding roots of photosynthetic plants. The atp1 gene was amplified by Sanger sequencing to determine the taxonomic affiliation of the mycoheterotrophic plants. A 280 bp region of the 18S rRNA gene of AMF was amplified with primers NS31/AML2 by high-throughput sequencing. The mycoheterotrophic specimens were assigned to V. parviflora with a bootstrap support of 72%. Glomus was the most abundant AMF genus, both in the mycoheterotrophic plants and in the litter and roots of photosynthetic plants. In addition, a few Glomus genotypes were abundantly enriched in the mycoheterotrophic plants, with only a few specimens colonized by Gigaspora, Acaulospora, and Scutellospora in a low proportion. These genotypes formed a cluster within a larger clade, suggesting that V. parviflora shows a preferential association with a narrow Glomus lineage which is not phylogenetically close to a previously identified V. parviflora?s associated lineage. Furthermore, detecting fungi from other families suggests that V. parviflora is colonized by other genera, although with low frequency. These findings provide new insights into the association between AMF and mycoheterotrophic species and highlight the importance of considering trap culture-independent approaches in understanding this symbiosis 653 $aAmazon 653 $aMetabarcoding 653 $aMycoheterotrophy 653 $aRbuscular mycorrhizal fungi 700 1 $aNOVAIS, C. B. de 700 1 $aDINIZ, P. P. 700 1 $aARAGÃO, O. O. da S. 700 1 $aSAGGIN JUNIOR, O. J. 700 1 $aJESUS, E. da C. 773 $tMycorrhiza$gv. 33, n. 4, p. 249 - 256, July 2023.
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Embrapa Agrobiologia (CNPAB) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
16/09/2005 |
Data da última atualização: |
30/09/2008 |
Autoria: |
MOREIRA, C. T.; BROGIN, R. L.; SOUZA, P. I. M. de; FARIAS NETO, A. L. de; ABUD, S.; TEIXEIRA, R. N. |
Título: |
Determinação da pureza varietal de sementes da cultivar de soja BRS Raimunda utilizando marcadores moleculares. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 27., 2005. Cornélio Procópio. Resumos... Londrina: Embrapa Soja, 2005. |
Páginas: |
p. 549-550. |
Descrição Física: |
1 CD-ROM. |
Série: |
(Embrapa Soja. Documentos, 257). |
Idioma: |
Português |
Notas: |
Organizado por Odilon Ferreira Saraiva, Janete Lasso Ortiz, Simone Ery Grosskopf. |
Thesagro: |
Marcador Molecular; Soja; Variedade. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00884naa a2200241 a 4500 001 1468377 005 2008-09-30 008 2005 bl uuuu u00u1 u #d 100 1 $aMOREIRA, C. T. 245 $aDeterminação da pureza varietal de sementes da cultivar de soja BRS Raimunda utilizando marcadores moleculares. 260 $c2005 300 $ap. 549-550.$c1 CD-ROM. 490 $a(Embrapa Soja. Documentos, 257). 500 $aOrganizado por Odilon Ferreira Saraiva, Janete Lasso Ortiz, Simone Ery Grosskopf. 650 $aMarcador Molecular 650 $aSoja 650 $aVariedade 700 1 $aBROGIN, R. L. 700 1 $aSOUZA, P. I. M. de 700 1 $aFARIAS NETO, A. L. de 700 1 $aABUD, S. 700 1 $aTEIXEIRA, R. N. 773 $tIn: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 27., 2005. Cornélio Procópio. Resumos... Londrina: Embrapa Soja, 2005.
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